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plko 1 shscr  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 shscr
    Plko 1 Shscr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+shscr/pLKO%2E1+shSCR+(Plasmid+%2317920)/pmc12622763-91-0-3
    Average 93 stars, based on 80 article reviews
    plko 1 shscr - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Targeting the Glucose–Insulin Link in Head and Neck Squamous Cell Carcinoma Induces Cytotoxic Oxidative Stress and Inhibits Cancer Growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Article Title: Targeting the glucose-insulin link in head and neck squamous cell carcinoma induces cytotoxic oxidative stress and inhibits cancer growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Control:

    Article Title: Targeting the Glucose–Insulin Link in Head and Neck Squamous Cell Carcinoma Induces Cytotoxic Oxidative Stress and Inhibits Cancer Growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Article Title: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape
    Article Snippet: .. An shRNA which does not recognize any known mammalian gene, pLKO.1-shSCR (Plasmid 1864) was used as a control (Addgene, Cambridge, MA). ..

    Article Title: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape.
    Article Snippet: .. An shRNAwhich does not recognize any known mammalian gene, pLKO.1-shSCR (Plasmid 1864) was used as a control (Addgene, Cambridge, MA). ..

    Article Title: Targeting the glucose-insulin link in head and neck squamous cell carcinoma induces cytotoxic oxidative stress and inhibits cancer growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Article Title: The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells.
    Article Snippet: TGF-β signaling mediates its biological effects by engaging canonical Smad proteins and crosstalking extensively with other signaling networks, including the NF-kB pathway.. The paracaspase MALT1 is an intracellular signaling molecule essential for NF-kB activation downstream of several key cell surface receptors.. Despite intensive research on TGF-β and NF-kB interactions, the significance of MALT1 in this context remains undecoded.

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    Plasmid Preparation:

    Article Title: Targeting the Glucose–Insulin Link in Head and Neck Squamous Cell Carcinoma Induces Cytotoxic Oxidative Stress and Inhibits Cancer Growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Article Title: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape
    Article Snippet: .. An shRNA which does not recognize any known mammalian gene, pLKO.1-shSCR (Plasmid 1864) was used as a control (Addgene, Cambridge, MA). ..

    Article Title: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape.
    Article Snippet: .. An shRNAwhich does not recognize any known mammalian gene, pLKO.1-shSCR (Plasmid 1864) was used as a control (Addgene, Cambridge, MA). ..

    Article Title: Targeting the glucose-insulin link in head and neck squamous cell carcinoma induces cytotoxic oxidative stress and inhibits cancer growth
    Article Snippet: For lentivirus production, HEK293T cells (RRID: CVCL_0063) were transfected with viral packaging plasmids psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID: Addgene_12259), and pLKO.1 (RRID: Addgene_139470) shRNA using Lipofectamine 3000 (Invitrogen). .. Cells were incubated with viral supernatant containing polybrene (8 mg/mL). pLKO.1-shScr (RRID: Addgene_17920) was used as the control vector. .. Targeting sequences for all shRNAs are provided in Supplementary Table S1. pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau and Paul Kaufman (Addgene, plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills and Kenneth Scott (RRID: Addgene_116103).

    Article Title: The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells.
    Article Snippet: TGF-β signaling mediates its biological effects by engaging canonical Smad proteins and crosstalking extensively with other signaling networks, including the NF-kB pathway.. The paracaspase MALT1 is an intracellular signaling molecule essential for NF-kB activation downstream of several key cell surface receptors.. Despite intensive research on TGF-β and NF-kB interactions, the significance of MALT1 in this context remains undecoded.

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    shRNA:

    Article Title: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape
    Article Snippet: .. An shRNA which does not recognize any known mammalian gene, pLKO.1-shSCR (Plasmid 1864) was used as a control (Addgene, Cambridge, MA). ..

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    Expressing:

    Article Title: The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells.
    Article Snippet: TGF-β signaling mediates its biological effects by engaging canonical Smad proteins and crosstalking extensively with other signaling networks, including the NF-kB pathway.. The paracaspase MALT1 is an intracellular signaling molecule essential for NF-kB activation downstream of several key cell surface receptors.. Despite intensive research on TGF-β and NF-kB interactions, the significance of MALT1 in this context remains undecoded.

    other:

    Article Title: CaMKII suppresses proteotoxicity by phosphorylating BAG3 in response to proteasomal dysfunction
    Article Snippet: pLKO.1-shScr , Addgene , 17920.



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